1 00:00:38,819 --> 00:00:36,750 taxi here and today if you know we have 2 00:00:41,389 --> 00:00:38,829 here John Dillinger there's a student 3 00:00:45,720 --> 00:00:41,399 with the chicken embryo determine and 4 00:00:48,210 --> 00:00:45,730 sponsor here mark duty who represents a 5 00:00:50,960 --> 00:00:48,220 Kentucky crime ticket and we appreciate 6 00:00:53,970 --> 00:00:50,970 all the support the crew gives us and 7 00:00:55,319 --> 00:00:53,980 we're happy to have the sponsor and the 8 00:00:57,899 --> 00:00:55,329 students here with that I'll turn it 9 00:01:00,180 --> 00:00:57,909 over to you John and you can tell us 10 00:01:02,970 --> 00:01:00,190 about your experience well I'm very 11 00:01:04,799 --> 00:01:02,980 excited to be here because it's really 12 00:01:07,650 --> 00:01:04,809 one of my dreams I've been working on 13 00:01:09,980 --> 00:01:07,660 for a long time when I started out in 14 00:01:12,210 --> 00:01:09,990 the ninth grade I thought to myself 15 00:01:14,790 --> 00:01:12,220 imagine this chicken egg in the back of 16 00:01:16,859 --> 00:01:14,800 the barn yard gravity's causing yoke to 17 00:01:19,050 --> 00:01:16,869 fall to the bottom of the egg that a hen 18 00:01:21,660 --> 00:01:19,060 has a natural instinct of turning over 19 00:01:23,249 --> 00:01:21,670 that egg around it's there for the yoga 20 00:01:24,570 --> 00:01:23,259 fault go back up to the top and gravity 21 00:01:27,749 --> 00:01:24,580 pulls it back down to the bottom again 22 00:01:29,430 --> 00:01:27,759 now what would happen to that egg up in 23 00:01:31,320 --> 00:01:29,440 space when you don't have gravity 24 00:01:33,810 --> 00:01:31,330 pulling that yoke down to the bottom how 25 00:01:37,080 --> 00:01:33,820 would that embryo develop from that 26 00:01:39,270 --> 00:01:37,090 basic basic hypothesis I developed a 27 00:01:40,789 --> 00:01:39,280 little broader range of what I'm trying 28 00:01:43,590 --> 00:01:40,799 to accomplish with my experience 29 00:01:46,020 --> 00:01:43,600 experiment since the ninth grade I've 30 00:01:48,270 --> 00:01:46,030 gone into what I consider four key areas 31 00:01:50,910 --> 00:01:48,280 of science that I'm looking for the 32 00:01:54,380 --> 00:01:50,920 first one is embryo development can we 33 00:01:58,469 --> 00:01:54,390 maintain embryo development up in space 34 00:02:00,450 --> 00:01:58,479 can-can an embryo develop up in space 35 00:02:02,370 --> 00:02:00,460 you know we can grow a better crystal in 36 00:02:05,940 --> 00:02:02,380 space is Earth the best place to have 37 00:02:08,370 --> 00:02:05,950 children the second area of interest 38 00:02:10,080 --> 00:02:08,380 that I'm looking at my project is one of 39 00:02:11,729 --> 00:02:10,090 bone calcium and I'm sure you're all 40 00:02:14,460 --> 00:02:11,739 aware of the bone loss that astronauts 41 00:02:15,630 --> 00:02:14,470 experience up in space for when they're 42 00:02:17,789 --> 00:02:15,640 up there for an extended period of time 43 00:02:19,979 --> 00:02:17,799 and what I'm going to do with my 44 00:02:23,400 --> 00:02:19,989 experiment is look at the mineralization 45 00:02:24,930 --> 00:02:23,410 and a demineralization process of the 46 00:02:27,180 --> 00:02:24,940 bone development and see how the 47 00:02:29,810 --> 00:02:27,190 calcification process occurs within the 48 00:02:32,310 --> 00:02:29,820 developing embryos of the chicken egg 49 00:02:34,680 --> 00:02:32,320 the third thing that I'm going to be 50 00:02:37,380 --> 00:02:34,690 looking at is what I call production 51 00:02:39,330 --> 00:02:37,390 performance I'm going to actually let 52 00:02:40,900 --> 00:02:39,340 some of the eggs hatch take them through 53 00:02:42,550 --> 00:02:40,910 the launch 54 00:02:45,040 --> 00:02:42,560 the space environment the landing and 55 00:02:46,210 --> 00:02:45,050 see what results okay can we have and 56 00:02:48,400 --> 00:02:46,220 they go through that type of environment 57 00:02:50,950 --> 00:02:48,410 and hatch what type of chicken is 58 00:02:53,290 --> 00:02:50,960 produced after it's been in that kind of 59 00:02:55,300 --> 00:02:53,300 environment and we can compare we can 60 00:02:57,670 --> 00:02:55,310 compare and see okay does it does the 61 00:03:00,040 --> 00:02:57,680 bird grow faster what type of blood 62 00:03:02,320 --> 00:03:00,050 quality does it have the semen 63 00:03:04,060 --> 00:03:02,330 production and all kinds of different 64 00:03:05,950 --> 00:03:04,070 areas of interest that we can look at as 65 00:03:08,770 --> 00:03:05,960 far as the production performance is 66 00:03:10,300 --> 00:03:08,780 concerned another area that we can look 67 00:03:12,430 --> 00:03:10,310 at is what i'd like to term the inner 68 00:03:14,560 --> 00:03:12,440 ear that's one that's responsible for 69 00:03:16,690 --> 00:03:14,570 your equilibrium and your motion 70 00:03:18,190 --> 00:03:16,700 sickness and we're going to look at the 71 00:03:20,740 --> 00:03:18,200 inner ear of the developing chicken 72 00:03:22,180 --> 00:03:20,750 embryo you see okay what kind of effect 73 00:03:23,920 --> 00:03:22,190 does this weightless environment have on 74 00:03:28,390 --> 00:03:23,930 the developing embryo and how the inner 75 00:03:30,940 --> 00:03:28,400 ear develops to obtain the optimum 76 00:03:33,340 --> 00:03:30,950 amount of data from this project I had 77 00:03:36,190 --> 00:03:33,350 to divide the age of the eggs into two 78 00:03:38,980 --> 00:03:36,200 groups it takes 21 days for an egg to 79 00:03:41,920 --> 00:03:38,990 hatch there be 16 eggs there are two 80 00:03:44,949 --> 00:03:41,930 days old maybe 16 eggs that are nine 81 00:03:48,340 --> 00:03:44,959 days old all the studies I have two 82 00:03:50,800 --> 00:03:48,350 identical hardware units all the studies 83 00:03:52,360 --> 00:03:50,810 will be done on a comparative basis the 84 00:03:55,480 --> 00:03:52,370 only variable that will be different as 85 00:03:57,699 --> 00:03:55,490 a weightless environment of space for 86 00:04:00,100 --> 00:03:57,709 this project I've composed a team of 87 00:04:02,199 --> 00:04:00,110 seven of the top scientists in their 88 00:04:05,580 --> 00:04:02,209 respective field two of them are from 89 00:04:08,770 --> 00:04:05,590 Purdue where I was from Tulane and and 90 00:04:11,140 --> 00:04:08,780 14 of them from Boston University they 91 00:04:14,710 --> 00:04:11,150 serve as my scientific advisors for this 92 00:04:16,690 --> 00:04:14,720 experiment as a mechanical engineering 93 00:04:20,110 --> 00:04:16,700 student I enjoy working with the 94 00:04:22,560 --> 00:04:20,120 hardware and the hardware we have given 95 00:04:26,680 --> 00:04:22,570 you all a folder and where we have a 96 00:04:29,080 --> 00:04:26,690 hardware we have outlined what we call a 97 00:04:30,909 --> 00:04:29,090 hardware description and I could let 98 00:04:32,020 --> 00:04:30,919 would just like to take you through some 99 00:04:34,870 --> 00:04:32,030 of the things that we've highlighted in 100 00:04:38,440 --> 00:04:34,880 that report and explain to you why the 101 00:04:39,760 --> 00:04:38,450 hardware is here and how it works the 102 00:04:42,310 --> 00:04:39,770 first thing we have is what we call the 103 00:04:45,659 --> 00:04:42,320 ecs is our environmental control system 104 00:04:48,700 --> 00:04:45,669 what this is composed of is we have an 105 00:04:51,760 --> 00:04:48,710 on-off controller it hooks up to the 106 00:04:54,640 --> 00:04:51,770 shuttle 28 volt DC power the controller 107 00:04:57,280 --> 00:04:54,650 is on the back side of the front plate 108 00:05:00,280 --> 00:04:57,290 we have five silicon rubber heater 109 00:05:02,590 --> 00:05:00,290 strips the on-off controller functions 110 00:05:05,020 --> 00:05:02,600 in conjunction with the silicon rubber 111 00:05:07,870 --> 00:05:05,030 heater strips that produce the uniform 112 00:05:10,150 --> 00:05:07,880 temperature by locating the heater 113 00:05:11,710 --> 00:05:10,160 strips in that location and by putting 114 00:05:14,200 --> 00:05:11,720 the fan of having to act like a ceiling 115 00:05:15,790 --> 00:05:14,210 fan we've created uniform temperature so 116 00:05:18,879 --> 00:05:15,800 that all the eggs are seeing the exact 117 00:05:22,360 --> 00:05:18,889 temperature we have what i call a 118 00:05:24,520 --> 00:05:22,370 humidity control system which is down 119 00:05:27,340 --> 00:05:24,530 below the fan on the bottom i have what 120 00:05:29,770 --> 00:05:27,350 is called a humidistat that senses the 121 00:05:32,680 --> 00:05:29,780 humidity on the inside of the incubator 122 00:05:34,629 --> 00:05:32,690 and it's an on-off controller when the 123 00:05:37,570 --> 00:05:34,639 humidity gets over a certain level it 124 00:05:40,689 --> 00:05:37,580 turns on an air pump located directly 125 00:05:44,200 --> 00:05:40,699 below squirrel that air pump circulates 126 00:05:46,390 --> 00:05:44,210 air through a desiccant tube so when the 127 00:05:48,760 --> 00:05:46,400 humidity gets over a certain level the 128 00:05:50,350 --> 00:05:48,770 humidistat turns on turns the air pump 129 00:05:52,270 --> 00:05:50,360 on circulates the air through the 130 00:05:54,969 --> 00:05:52,280 desiccant the moisture is removed and 131 00:05:57,070 --> 00:05:54,979 when the air humidity in the air gets 132 00:05:59,409 --> 00:05:57,080 below the desired level that it'll shut 133 00:06:01,480 --> 00:05:59,419 off and it will maintain a uniform 134 00:06:03,610 --> 00:06:01,490 humidity because what we're trying to do 135 00:06:04,930 --> 00:06:03,620 duplicate the conditions that the hen 136 00:06:06,610 --> 00:06:04,940 provides in the back of the barn yard 137 00:06:08,500 --> 00:06:06,620 and the normal humidity would be 138 00:06:15,760 --> 00:06:08,510 sixty-five percent and a temperature 139 00:06:19,420 --> 00:06:15,770 would be 99.5 okay I've put in a time 140 00:06:21,370 --> 00:06:19,430 line within the crew integration the 141 00:06:24,730 --> 00:06:21,380 time line starts when we load the 142 00:06:26,320 --> 00:06:24,740 incubator in the laboratory the first 143 00:06:30,159 --> 00:06:26,330 career interaction would take place at 144 00:06:32,649 --> 00:06:30,169 24 to 30 hours the first thing we want 145 00:06:34,600 --> 00:06:32,659 to do is what we call a unit operations 146 00:06:37,390 --> 00:06:34,610 check and that's nothing more than 147 00:06:41,080 --> 00:06:37,400 making sure that the switch is on that 148 00:06:42,700 --> 00:06:41,090 the power on light is on and just 149 00:06:48,010 --> 00:06:42,710 checking the system out to make sure the 150 00:06:50,790 --> 00:06:48,020 fan is running the system is running the 151 00:06:54,159 --> 00:06:50,800 second thing that needs to be done is 152 00:06:56,110 --> 00:06:54,169 temperature and humidity recordings now 153 00:06:59,200 --> 00:06:56,120 this will be done with the squirrel 154 00:07:00,700 --> 00:06:59,210 meter longer and as you can see there's 155 00:07:02,860 --> 00:07:00,710 three buttons on the squirrel million 156 00:07:04,930 --> 00:07:02,870 logger a B and C like I've outlined in 157 00:07:08,410 --> 00:07:04,940 the integration plan 158 00:07:11,130 --> 00:07:08,420 button a will be pushed once which will 159 00:07:15,370 --> 00:07:11,140 bring up the display if button a is 160 00:07:17,620 --> 00:07:15,380 pushed again and I have to be pushed the 161 00:07:21,040 --> 00:07:17,630 second time it will come up and it'll 162 00:07:24,750 --> 00:07:21,050 say 21 and there at that point in time 163 00:07:27,760 --> 00:07:24,760 this first temperature can be recorded 164 00:07:29,230 --> 00:07:27,770 when button be is pushed there's four 165 00:07:30,790 --> 00:07:29,240 channels because there's two humidity 166 00:07:34,720 --> 00:07:30,800 probes and there's two temperature 167 00:07:37,480 --> 00:07:34,730 probes and once you put push button a to 168 00:07:39,100 --> 00:07:37,490 get it to channel 2 and then button be 169 00:07:41,260 --> 00:07:39,110 will be pushed to switch from channel 170 00:07:42,880 --> 00:07:41,270 from the first temperature second 171 00:07:44,380 --> 00:07:42,890 temperature first humidity second 172 00:07:47,560 --> 00:07:44,390 humidity and that's a continuous cycle 173 00:07:48,880 --> 00:07:47,570 you can sit here and push button be like 174 00:07:50,680 --> 00:07:48,890 for example there's humidity there's 175 00:07:53,230 --> 00:07:50,690 humidity temperature temperature and you 176 00:07:55,690 --> 00:07:53,240 can just go around the clock rotating 177 00:07:59,020 --> 00:07:55,700 temperature and humidity before just by 178 00:08:00,310 --> 00:07:59,030 pushing button number B button number C 179 00:08:03,370 --> 00:08:00,320 will not have to be used at all that's 180 00:08:06,460 --> 00:08:03,380 de strictly to program the squirrel meat 181 00:08:08,230 --> 00:08:06,470 logger oh yeah are you manually 182 00:08:09,220 --> 00:08:08,240 recording the temperatures or is it when 183 00:08:12,430 --> 00:08:09,230 you say you record the temperature 184 00:08:17,710 --> 00:08:12,440 you're you're actually recording it in 185 00:08:20,530 --> 00:08:17,720 your squirrel meter right as the neg 186 00:08:23,650 --> 00:08:20,540 develops the response wash so therefore 187 00:08:26,770 --> 00:08:23,660 as the mission increases the amount of 188 00:08:29,500 --> 00:08:26,780 oxygen consumption increases that's why 189 00:08:30,850 --> 00:08:29,510 the crew interactions become at a closer 190 00:08:32,620 --> 00:08:30,860 interval towards the end of the mission 191 00:08:34,959 --> 00:08:32,630 whereas at the beginning of the mission 192 00:08:36,510 --> 00:08:34,969 it's not the incubator does not have to 193 00:08:38,800 --> 00:08:36,520 be attended to quite as frequently 194 00:08:40,810 --> 00:08:38,810 because of the oxygen consumption the 195 00:08:43,150 --> 00:08:40,820 eggs are getting larger required more 196 00:08:45,130 --> 00:08:43,160 oxygen so I had the crew interaction 197 00:08:47,530 --> 00:08:45,140 what you have to do is open up the hatch 198 00:08:54,840 --> 00:08:47,540 door this is done just strictly by 199 00:09:05,080 --> 00:09:01,150 removing the hat store it's tethered 10 200 00:09:06,760 --> 00:09:05,090 what I call capillary pads the first pad 201 00:09:09,160 --> 00:09:06,770 will be removed at the first crew 202 00:09:12,250 --> 00:09:09,170 interaction the pad will be opened up 203 00:09:15,010 --> 00:09:12,260 and exchange this will be this packet 204 00:09:18,670 --> 00:09:15,020 will be airtight and pre charged with 205 00:09:20,980 --> 00:09:18,680 about five CCS moisture the pad will be 206 00:09:22,810 --> 00:09:20,990 exchanged this one will be taken off the 207 00:09:26,410 --> 00:09:22,820 back of the hat store put back on the 208 00:09:27,670 --> 00:09:26,420 side and the fresh one we takin off open 209 00:09:34,660 --> 00:09:27,680 up and put it on the back of the hatch 210 00:09:43,750 --> 00:09:34,670 store after the five minutes the hat 211 00:09:50,300 --> 00:09:47,720 and is it going to affect the moisture 212 00:09:53,870 --> 00:09:50,310 content of that if you I assume you 213 00:09:56,420 --> 00:09:53,880 prefer to leave the putting the ad until 214 00:09:58,490 --> 00:09:56,430 the last right right just before you 215 00:10:00,890 --> 00:09:58,500 right close it up before the before the 216 00:10:02,960 --> 00:10:00,900 hash stores closed back up and then what 217 00:10:04,190 --> 00:10:02,970 that will do is that will recharge the 218 00:10:06,980 --> 00:10:04,200 system with the right amount of moisture 219 00:10:09,140 --> 00:10:06,990 and then the humidity control system 220 00:10:11,620 --> 00:10:09,150 will compensate if it goes over you know 221 00:10:13,670 --> 00:10:11,630 goes under that will keep it in line 222 00:10:18,320 --> 00:10:13,680 it's all humidity you're trying to 223 00:10:27,950 --> 00:10:18,330 maintain a 65 person right what the legs 224 00:10:29,750 --> 00:10:27,960 doing Houston 80 any questions that 225 00:10:32,360 --> 00:10:29,760 you'd like to ask her let me ask a dumb 226 00:10:35,300 --> 00:10:32,370 question Jenna I'll get it from the kids 227 00:10:38,510 --> 00:10:35,310 if you put the eggs in two days before 228 00:10:41,060 --> 00:10:38,520 launch right where do you get the eggs 229 00:10:42,650 --> 00:10:41,070 man well we have we talked in Florida 230 00:10:44,390 --> 00:10:42,660 eggs yeah we're talking Florida eggs 231 00:10:46,010 --> 00:10:44,400 what happens is there's a lot of 232 00:10:48,800 --> 00:10:46,020 districts involved in trying to have two 233 00:10:50,840 --> 00:10:48,810 different age group of eggs we have a 234 00:10:53,060 --> 00:10:50,850 supplier to kentucky fried chicken at 235 00:10:55,250 --> 00:10:53,070 live oak florida which is called cold 236 00:10:57,350 --> 00:10:55,260 kiss hatcheries and they have rooms full 237 00:10:59,900 --> 00:10:57,360 of eggs probably the size of this room 238 00:11:03,110 --> 00:10:59,910 right here and what we will do is we 239 00:11:05,750 --> 00:11:03,120 have them set back eggs like starting 10 240 00:11:08,810 --> 00:11:05,760 days before the mission so that they 241 00:11:10,460 --> 00:11:08,820 have eggs at every day from ten days on 242 00:11:12,530 --> 00:11:10,470 a case there be any kind of delay and 243 00:11:15,080 --> 00:11:12,540 what we do is we have designed and built 244 00:11:16,700 --> 00:11:15,090 a portable generator an incubator system 245 00:11:19,340 --> 00:11:16,710 to carry the eggs from live oak florida 246 00:11:22,310 --> 00:11:19,350 down to the cape then we'll load them up 247 00:11:24,890 --> 00:11:22,320 in hangar l and it'll be loaded like 12 248 00:11:26,120 --> 00:11:24,900 2 hours prior to flight what you wants 249 00:11:27,950 --> 00:11:26,130 to record the temperatures and humidity 250 00:11:29,930 --> 00:11:27,960 rights right before we open it right 251 00:11:31,010 --> 00:11:29,940 would it not be interesting since you 252 00:11:34,030 --> 00:11:31,020 really don't know what's gonna go in 253 00:11:36,080 --> 00:11:34,040 there to record them after you close it 254 00:11:37,250 --> 00:11:36,090 sure so yeah that would really want 255 00:11:38,720 --> 00:11:37,260 understand how the brain works from the 256 00:11:39,920 --> 00:11:38,730 engineering strike point you really 257 00:11:42,860 --> 00:11:39,930 getting right here for how much mass 258 00:11:44,240 --> 00:11:42,870 exchange right that'd be great and I 259 00:11:46,470 --> 00:11:44,250 just mean as long as you're right in the 260 00:11:50,160 --> 00:11:46,480 mini when there's that when this way you 261 00:11:51,329 --> 00:11:50,170 all right i mean i don't know it's 262 00:11:56,240 --> 00:11:51,339 probably not i mean as long as you're 263 00:12:00,720 --> 00:11:56,250 doing it channel 2 second temperature 264 00:12:03,750 --> 00:12:00,730 channel 3 first humidity channel 4 2nd 265 00:12:05,430 --> 00:12:03,760 humidity third channel that is that is 266 00:12:06,960 --> 00:12:05,440 if you want to play back at that's 267 00:12:08,879 --> 00:12:06,970 that's something you don't have to worry 268 00:12:10,560 --> 00:12:08,889 about this kind of get a feel this was 269 00:12:12,210 --> 00:12:10,570 from a previous testimony right in other 270 00:12:18,030 --> 00:12:12,220 words that's all the temperatures that 271 00:12:20,340 --> 00:12:18,040 the incubator would be seen that's the 272 00:12:26,610 --> 00:12:20,350 time now we call on the column first 273 00:12:28,710 --> 00:12:26,620 number to a channel and then the motive 274 00:12:31,379 --> 00:12:28,720 yeah samode this would be a modem this 275 00:12:33,990 --> 00:12:31,389 video's Netanyahu channel them yeah you 276 00:12:36,660 --> 00:12:34,000 go modes 13 17 angles would have one 277 00:12:39,569 --> 00:12:36,670 through four actually there's nine modes 278 00:12:42,569 --> 00:12:39,579 yeah there's nine okay yeah those are 279 00:12:44,009 --> 00:12:42,579 kinds yeah tues on Jeffrey's on Channel 280 00:12:45,720 --> 00:12:44,019 Four's on but that's even open your 281 00:12:48,620 --> 00:12:45,730 thickness that'll be will have that done 282 00:12:52,319 --> 00:12:48,630 before we start and then first channel 283 00:12:56,100 --> 00:12:52,329 it'll flash off and that's all right 284 00:12:58,319 --> 00:12:56,110 it's all so sure do it you're no idea 285 00:12:59,879 --> 00:12:58,329 tree again before you turn it on kid 286 00:13:02,490 --> 00:12:59,889 right did you know go on channel 287 00:13:06,050 --> 00:13:02,500 whatever the write up is always right I 288 00:13:08,040 --> 00:13:06,060 did it again that's really hard right 289 00:13:09,329 --> 00:13:08,050 well what you want to do is once you 290 00:13:15,540 --> 00:13:09,339 call it up then you want to push it 291 00:13:16,800 --> 00:13:15,550 again otherwise just gonna disappear go 292 00:13:22,519 --> 00:13:16,810 ahead try it i think you get a better 293 00:13:25,819 --> 00:13:24,170 now you gotta hit it wallah just play 294 00:13:27,679 --> 00:13:25,829 again right in Christ chance if we could 295 00:13:32,480 --> 00:13:27,689 build no but I what's the best I was mad 296 00:13:37,929 --> 00:13:32,490 as I say the hand on a step include man 297 00:13:45,949 --> 00:13:40,549 now see go ahead and push that again and 298 00:13:47,150 --> 00:13:45,959 you see it goes back to channel one yeah 299 00:13:48,769 --> 00:13:47,160 you could yeah you can all this will 300 00:13:51,199 --> 00:13:48,779 sound pro right right you just won't 301 00:13:53,179 --> 00:13:51,209 disappear right exactly once you get out 302 00:13:59,809 --> 00:13:53,189 of mode 1 it's gonna stay whatever motor 303 00:14:03,499 --> 00:13:59,819 ever and you see that piece of velcro 304 00:14:05,030 --> 00:14:03,509 would be going this way right right dope 305 00:14:06,949 --> 00:14:05,040 on the outside of the leg and then 306 00:14:10,790 --> 00:14:06,959 there's about girl on the sponge exactly 307 00:14:17,960 --> 00:14:10,800 this one does not happen because we want 308 00:14:20,150 --> 00:14:17,970 to save the anessa proof help grow any 309 00:14:21,739 --> 00:14:20,160 particular wine this region not really 310 00:14:24,049 --> 00:14:21,749 what we've done and we can maybe market 311 00:14:25,340 --> 00:14:24,059 if you guys would prefer but we've put 312 00:14:26,990 --> 00:14:25,350 that towards the bottom corner the other 313 00:14:29,179 --> 00:14:27,000 yeah you know you were in lung 314 00:14:30,980 --> 00:14:29,189 concentration yeah we thought you wanted 315 00:14:32,059 --> 00:14:30,990 them where no it's just it's just 316 00:14:47,590 --> 00:14:32,069 thought you know just to keep 317 00:15:07,340 --> 00:15:03,799 we won't change that sure I'll just tell 318 00:15:10,040 --> 00:15:07,350 you yeah we anybody at any time and no